TJGS - Olympia oyster transcriptome v2

combining….

reads from version 1

OA experiment data, multi levels

SE 36 bp gonad

Version 1 raw reads

http://eagle.fish.washington.edu/cnidarian/filtered_400_NoIndex_L006_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_2000_NoIndex_L007_R1.fastq.gz

OA larval data

http://eagle.fish.washington.edu/cnidarian/filtered_400_GTCCGC_L004_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_1600_GTGGCC_L004_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_1000_GTGAAA_L004_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_2200_GTTTCG_L004_R1.fastq.gz

SE Gonad data

http://eagle.fish.washington.edu/cnidarian/filtered_106A_Female_Mix_GATCAG_L007_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_106A_Male_Mix_TAGCTT_L007_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_108A_Male_Mix_AGTCAA_L007_R1.fastq.gz

http://eagle.fish.washington.edu/cnidarian/filtered_108A_Female_Mix_GGCTAC_L007_R1.fastq.gz

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Quality trimmed data

Remove duplicates

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de novo assembly

version 2.1

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version 2.2

how do assess best assembly